Expertises

The Bioprofiler resource of the Metabolism Platform performs qualitative and quantitative analyses of biomolecules. The activities offered include:

• Detection and quantification of physiological markers (peptides, metabolites, catecholamines, etc.)
• Characterization of enzymatic activities: inhibitor screening assays, energy metabolism, epigenetic signaling, etc.
• Analysis of therapeutic and toxic compounds: endocrine disruptors, bisphenols, aromatic amines, etc.
• Evaluation of the oxidative potential of nanoparticles

Equipments

• 5 HPLC systems, including 3 UFLC systems equipped with the following detection modules: UV/vis, photodiode array (PDA), fluorimetry, electrochemical detection, evaporative light scattering detection (ELSD), and mass spectrometry
• 3 gas chromatographs: GC-FID, GC-MS, and GC-MS/MS

 Analytical capacity

• Collaborative projects and service provision: Several thousand samples analyzed per year
• Involvement in multiple research projects (ANR, ANSES, NIH, European H2020 programs, etc.)

Training

Active participation in training activities (PhD students, postdoctoral fellows, researchers), as well as initial and continuing education programs:

• Master 1 Toxicology
• Master 2 BMC

Recent publications

Michail C, Berthelet J, Mechaly AE, Bui LC, Yang H, Cai D, Al Mahi A, Xie A, Bisio V, Sirri V, Dupret JM, Guidez F, Xu X, Joly N, Regad L, Viguier M, Deshayes F, Dulphy N, Green MR, Haouz A, Rodrigues Lima F.  The SETD2 L1609P mutation found in leukemia disrupts methyltransferase activity and reduces histone H3K36 trimethylation. J Biol Chem. 2026 Feb 5:111259. doi: 10.1016/j.jbc.2026.111259.

Onimus O, Arrivet F, Le Borgne T, Perez S, Castel J, Ansoult A, Bertrand B, Mashhour N, de Almeida C, Bui LC, Vandecasteele M, Luquet S, Venance L, Heck N, Marti F, Gangarossa G. The gut-brain vagal axis governs mesolimbic dopamine dynamics and reward events. Sci Adv. 2026 Jan 30;12(5): eadz0828. doi: 10.1126/sciadv.adz 0828.

Cui G, Ley M, Mechaly AE, Bui LC, Michail C, Berthelet J, Dairou J, Yang H, Chevreux G, Moroy G, Green MR, Haouz A, Rodrigues Lima F. Structural and functional characterization of CREB-binding protein (CREBBP) as a histone propionyltransferase. J Biol Chem. 2025 Aug;301(8):110444. doi: 10.1016/j.jbc.2025.110444. Epub 2025 Jul 2. PMID: 40615044

Deval G, Mawas S, Devineau S, Borderie D, Mikolajczak C, Lefèvre G, Bui LC, Renault J, Izabelle C, Fournier T, Ferecatu I, Boland S. Effects of co-exposure to benzo-a-pyrene and cerium dioxide nanoparticles on human lung and placental barriers. J Hazard Mater Adv. (2025) 19, 100802.

Jeanpierre M, Cognard J, Tusseau M, Riller Q, Bui LC, Berthelet J, Laurent A, Crickx E, Parlato M, Stolzenberg MC, Suarez F, Leverger G, Aladjidi N, Collardeau-Frachon S, Pietrement C, Malphettes M, Froissart A, Bole-Feysot C, Cagnard N, Rodrigues Lima F, Walzer T, Rieux-Laucat F, Belot A, Mathieu AL. Haploinsufficiency in PTPN2 leads to early-onset systemic autoimmunity from Evans syndrome to lupus. J Exp Med. 2024 Sep 2;221(9):e20232337. doi: 10.1084/jem.20232337. Epub 2024 Jul 19. PMID: 39028869

Delabar JM, Gomes MAGB, Fructuoso M, Sarrazin N, George N, Fleary-Roberts N, Sun H, Bui LC, Rodrigues-Lima F, Janel N, Dairou J, Maria EJ, Dodd RH, Cariou K, Potier MC. EGCG-like non-competitive inhibitor of DYRK1A rescues cognitive defect in a down syndrome model. Eur J Med Chem. 2024 Feb 5;265:116098. doi: 10.1016/j.ejmech.2023.116098. Epub 2024 Jan 1. PMID: 38171148

Le Coadou L, Berthelet J, Mechaly AE, Michail C, Bui LC, Dairou J, Haouz A, Dupret JM, Rodrigues Lima F. Structural and enzymatic evidence for the methylation of the ACK1 tyrosine kinase by the histone lysine methyltransferase SETD2. Biochem Biophys Res Commun. 2024 Feb 5;695:149400. doi: 10.1016/j.bbrc.2023.149400. Epub 2023 Dec 21. PMID: 38160530

Herrera Moro Chao D, Kirchner MK, Pham C, Foppen E, Denis RGP, Castel J, Morel C, Montalban E, Hassouna R, Bui LC, Renault J, Mouffle C, García-Cáceres C, Tschöp MH, Li D, Martin C, Stern JE, Luquet SH.Hypothalamic astrocytes control systemic glucose metabolism and energy balance. Cell Metab. 2022 Oct 4;34(10):1532-1547.

Liu R, Zhang W, Gou P, Berthelet J, Nian Q, Chevreux G, Legros V, Moroy G, Bui LC, Wang L, Dupret JM, Deshayes F, Rodrigues Lima F. Cisplatin causes covalent inhibition of protein-tyrosine phosphatase 1B (PTP1B) through reaction with its active site cysteine: Molecular, cellular and in vivo mice studies. Biomed Pharmacother. 2022 Sep;153:113372. doi: 10.1016/j.biopha.2022.113372.

Liu R, Sun Y, Berthelet J, Bui LC, Xu X, Viguier M, Dupret JM, Deshayes F, Rodrigues Lima F. Biochemical, Enzymatic, and Computational Characterization of Recurrent Somatic Mutations of the Human Protein Tyrosine Phosphatase PTP1B in Primary Mediastinal B Cell Lymphoma. Int J Mol Sci. 2022 Jun 24;23(13):7060. doi: 10.3390/ijms23137060.

Berthelet J, Michail C, Bui LC, Le Coadou L, Sirri V, Wang L, Dulphy N, Dupret JM, Chomienne C, Guidez F, Rodrigues-Lima F. The Benzene Hematotoxic and Reactive Metabolite 1,4-Benzoquinone Impairs the Activity of the Histone Methyltransferase SET Domain Containing 2 (SETD2) and Causes Aberrant Histone H3 Lysine 36 Trimethylation (H3K36me3). Mol Pharmacol. 2021 Sep;100(3):283-294.

Zhang W, Berthelet J, Michail C, Bui LC, Gou P, Liu R, Duval R, Renault J, Dupret JM, Guidez F, Chomienne C, Rodrigues Lima F. Human CREBBP acetyltransferase is impaired by etoposide quinone, an oxidative and leukemogenic metabolite of the anticancer drug etoposide through modification of redox-sensitive zinc-finger cysteine residues. Free Radic Biol Med. 2021 Jan; 162:27-37.

Crobeddu B, Baudrimont I, Deweirdt J, Jean Sciare, Anne Badel, Anne-Claude Camproux ,  BUI LC,  Armelle Baeza-Squiban. Lung Antioxidant Depletion: A Predictive Indicator of Cellular Stress Induced by Ambient Fine Particles. Environ Sci Technol. 2020;54(4):2360‐2369.

Recent Projects

2024-2026 LNCC -Impacts moléculaires, cellulaires et structuraux de mutations récurrentes de la protéine tyrosine phosphatase TC-PTP identifiées dans des cancers humains

2022-2027 ANR – Micromethyl:Microtubule methylation by SET domain protein methyltransferases

2022-2027 NIH R01 – Identifying/Targeting Mechanisms of Lymphomagenesis Driven by CREBBP Inactivation

2021-2024 EU H2020 – PlasticsFatE: Plastics Fate and Effects in the human body

2020-2025 – ANR -APIMAMA: Air pollution mitigation actions for megacities in Africa

2018-2025 – Projet MONORTHO “Evaluation de la libération de monomères de résines composites de collage en orthodontie”

Recent partnerships

UMR-S 1111 INSERM, UMR 5308 – CNRS, ENS de Lyon Centre International de Recherche en Infectiologie

UMR-S 1185 INSERM Signalisation Hormonale, Physiopathologie Endocrinienne et Métabolique

U1266 INSERM – Institut de Psychiatrie et Neurosciences de Paris (IPNP)

UMR-S 1138 Inserm Laboratoire Physiopathologie orale moléculaire

UMR7216 Epigénétiques et Destin cellulaire

Unité de Biologie Fonctionnelle et Adaptative CNRS UMR 8251

EPPLEY Institute

Department of Pharmacology & Toxicology, University of Toronto, Canada

Department of Lymphoma and Myeloma, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.